However, recent advances have made it possible to manipulate photosynthesis using molecular genetic technology Andrews and WhineyRaines For example, measurements of photosynthetic efficiency quantum yield at different wavelengths of light Emerson and Lews led to the insight that two distinct forms of Chl must be excited in oxygenic photosynthesis.
A split-beam difference spectrophotometer.
In this study of the model Synechocystis sp. Towards a functional dissection of thioredoxin networks in plant cells. Mutants defective in pheophorbide a oxygenase, which converts phephorbide a to open tetrapyrrole, accumulate pheophorbide a and display cell death in a light-dependent manner.
Photosynthesis also generates reactive oxygen species, which are now appreciated as being regulatory factors for many biological processes rather than inevitable by-products of photosynthesis Wagner et al.
Acclimation of tobacco leaves to high light intensity drives the plastoquinone oxidation system—relationship among the fraction of open PSII centers, non-photochemical quenching of Chl fluorescence and the maximum quantum yield of PSII in the dark.
Improving the efficiency of photosynthesis by artificial modification of photosynthetic proteins and pathways has long been considered impossible or at best problematic, because, over evolutionary time, photosynthesis has become complex and tightly regulated.